验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18042-1-AP targets CCNY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | CCNY fusion protein Ag12674 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin Y |
| Synonyms | Cyclin fold protein 1, Cyclin box protein 1, Cyc Y, CFP1, CBCP1 |
| Calculated Molecular Weight | 33 kDa, 39 kDa |
| Observed Molecular Weight | 33-39 kDa |
| GenBank Accession Number | BC104773 |
| Gene Symbol | CCNY |
| Gene ID (NCBI) | 219771 |
| RRID | AB_2074004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8ND76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCNY antibody 18042-1-AP | Download protocol |
| IF protocol for CCNY antibody 18042-1-AP | Download protocol |
| IP protocol for CCNY antibody 18042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Prog Neurobiol Cyclin Y, a novel actin-binding protein, regulates spine plasticity through the cofilin-actin pathway. | ||
Prog Neurobiol Myristoylation-dependent palmitoylation of cyclin Y modulates long-term potentiation and spatial learning | ||
Pharmacol Res Ccny knockout mice display an enhanced susceptibility to kainic acid-induced epilepsy.
| ||
Biochem J Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16. | ||
Cells The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis. | ||