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CCNY Polyclonal antibody

CCNY Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 18042-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (2)

Cyclin fold protein 1, Cyclin box protein 1, Cyc Y, CFP1, CBCP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18042-1-AP targets CCNY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 CCNY fusion protein Ag12674 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin Y
别名 Cyclin fold protein 1, Cyclin box protein 1, Cyc Y, CFP1, CBCP1
计算分子量 33 kDa, 39 kDa
观测分子量 33-39 kDa
GenBank蛋白编号BC104773
基因名称 CCNY
Gene ID (NCBI) 219771
RRIDAB_2074004
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8ND76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.

实验方案

Product Specific Protocols
WB protocol for CCNY antibody 18042-1-APDownload protocol
IF protocol for CCNY antibody 18042-1-APDownload protocol
IP protocol for CCNY antibody 18042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Prog Neurobiol

Cyclin Y, a novel actin-binding protein, regulates spine plasticity through the cofilin-actin pathway.

Authors - Hongik Hwang
human,mouseWB,IP

Prog Neurobiol

Myristoylation-dependent palmitoylation of cyclin Y modulates long-term potentiation and spatial learning

Authors - Jiyeon Seo
mouseWB

Pharmacol Res

Ccny knockout mice display an enhanced susceptibility to kainic acid-induced epilepsy.

Authors - Hongik Hwang
  • KO Validated
mouseWB

Biochem J

Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16.

Authors - Saifeldin N Shehata
humanWB,IF

Cells

The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis.

Authors - Andrea Molinas
mouseWB, IP

Cell Signal

Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase.

Authors - Shehata Saifeldin N SN
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