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  • Featured Product
  • KD/KO Validated

CCNY Monoclonal antibody

CCNY Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2C9E3

Cat no : 66865-1-Ig

Print datasheet

Synonyms

C10orf9, CBCP1, CCNY, CFP1, Cyc Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin X, cyclin Y, FLJ95513

验证数据展示

  • WB analysis using 66865-1-Ig

    WB analysis using 66865-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66865-1-Ig (CCNY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 66865-1-Ig

    WB analysis of A549 using 66865-1-Ig

    WB result of CCNY antibody (66865-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNY transfected A549 cells.

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66865-1-Ig targets CCNY in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human, Rat, Mouse
Cited Reactivitymouse
Immunogen CCNY fusion protein Ag12691 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin Y
Synonyms C10orf9, CBCP1, CCNY, CFP1, Cyc Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin X, cyclin Y, FLJ95513
Calculated Molecular Weight 33 kDa, 39 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC104773
Gene Symbol CCNY
Gene ID (NCBI) 219771
RRIDAB_2882202
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8ND76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.

实验方案

Product Specific Protocols
WB protocol for CCNY antibody 66865-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy.

Authors - Marc Dohmen
View Article