CCR2b-specific Polyclonal antibody
CCR2b-specific Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16154-1-AP targets CCR2b-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chemokine (C-C motif) receptor 2 |
| Synonyms | CCR2, CC-CKR-2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2 |
| Calculated Molecular Weight | 41 kDa |
| GenBank Accession Number | NM_000648 |
| Gene Symbol | CCR2 |
| Gene ID (NCBI) | 729230 |
| RRID | AB_2878224 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16154-1-AP is raised against the peptide of 344-360 amino acids of CCR2b. It is specific to CCR2b.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCR2b-specific antibody 16154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Tissue-resident, extravascular Ly6c- monocytes are critical for inflammation in the synovium | ||
Int J Mol Sci Inducing Receptor Degradation as a Novel Approach to Target CC Chemokine Receptor 2 (CCR2) | ||
Cancer Med Combination therapy with bevacizumab and a CCR2 inhibitor for human ovarian cancer: An in vivo validation study |