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  • Featured Product
  • KD/KO Validated

CCS Monoclonal antibody, PBS Only

CCS Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3F5E5

Cat No : 68341-1-PBS

Print datasheet

Synonyms

CCS

验证数据展示

  • WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    WB analysis using 68341-1-Ig (same clone as 68341-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB analysis of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells. This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    IF Staining of HepG2 using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    IHC staining of mouse brain using 68341-1-Ig (same clone as 68341-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68341-1-PBS in a different storage buffer formulation.

产品信息

68341-1-PBS targets CCS in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen CCS fusion protein Ag18860 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name copper chaperone for superoxide dismutase
Synonyms CCS
Calculated Molecular Weight 274 aa, 29 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC105016
Gene Symbol CCS
Gene ID (NCBI) 9973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14618
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.