• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CCT3 Polyclonal antibody

CCT3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10571-1-AP

Print datasheet

Synonyms

TCP 1 gamma, hTRiC5, Chaperonin containing T-complex polypeptide 1 subunit 3, CCT-gamma, CCTgamma

验证数据展示

  • WB analysis using 10571-1-AP

    WB analysis using 10571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10571-1-AP

    IP experiment of mouse brain using 10571-1-AP

    IP result of anti-CCT3 (IP:10571-1-AP, 3ug; Detection:10571-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

  • IHC staining of human liver cancer using 10571-1-AP

    IHC staining of human liver cancer using 10571-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 10571-1-AP

    IHC staining of human liver cancer using 10571-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10571-1-AP (CCT3 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 10571-1-AP

    IF Staining of HepG2 using 10571-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10571-1-AP (CCT3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 10571-1-AP

    WB analysis using 10571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, rat kidney tissue, HeLa cells, Jurkat cells, U2OS cells, mouse brain tissue, rat brain tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10571-1-AP targets CCT3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CCT3 fusion protein Ag0843 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 3 (gamma)
Synonyms TCP 1 gamma, hTRiC5, Chaperonin containing T-complex polypeptide 1 subunit 3, CCT-gamma, CCTgamma
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC006501
Gene Symbol CCT3
Gene ID (NCBI) 7203
RRIDAB_2073658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49368
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCT3 antibody 10571-1-APDownload protocol
IHC protocol for CCT3 antibody 10571-1-APDownload protocol
IF protocol for CCT3 antibody 10571-1-APDownload protocol
IP protocol for CCT3 antibody 10571-1-APDownload protocol
FC protocol for CCT3 antibody 10571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Genet

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Authors - Tarkar Aarti A
View Article
mouseWB

Cell

Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.

Authors - Erik McShane
View Article
human,mouseWB

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S
  • KD Validated
View Article
humanWB

J Cell Biol

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Authors - Yu-Cheng Zhang
View Article
humanWB,IF

Dev Biol

Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae.

Authors - Redgrove Kate A KA
View Article
humanWB

Toxicol Appl Pharmacol

Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1.

Authors - Hongyang Shi
  • KD Validated
View Article