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CCT4 Monoclonal antibody, PBS Only

CCT4 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rabbit, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C6A6

Cat No : 67455-1-PBS

Print datasheet

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

验证数据展示

  • WB analysis using 67455-1-Ig (same clone as 67455-1-PBS)

    WB analysis using 67455-1-Ig (same clone as 67455-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67455-1-Ig (CCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67455-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67455-1-Ig (same clone as 67455-1-PBS)

    IF Staining of U2OS using 67455-1-Ig (same clone as 67455-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT4 antibody (67455-1-Ig, Clone: 3C6A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67455-1-PBS in a different storage buffer formulation.

产品信息

67455-1-PBS targets CCT4 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rabbit, Pig samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rabbit, Pig
Immunogen CCT4 fusion protein Ag17084 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 4 (delta)
Synonyms CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta
Calculated Molecular Weight 539 aa, 58 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC106934
Gene Symbol CCT4
Gene ID (NCBI) 10575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50991
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.