• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67400-1-Ig (same clone as 67400-1-PBS)

    WB analysis using 67400-1-Ig (same clone as 67400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67400-1-Ig (CCT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67400-1-Ig (same clone as 67400-1-PBS)

    IHC staining of human skin cancer using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67400-1-Ig (CCT5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67400-1-Ig (same clone as 67400-1-PBS)

    WB analysis of HEK-293 using 67400-1-Ig (same clone as 67400-1-PBS)

    WB result of CCT5 antibody (67400-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells. This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67400-1-Ig (same clone as 67400-1-PBS)

    IF Staining of U2OS using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CCT5 Monoclonal antibody, PBS Only

CCT5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67400-1-PBS

产品说明书

CloneNo. 1B12E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

67400-1-PBS targets CCT5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCT5 fusion protein Ag21421 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 5 (epsilon)
别名 KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon
计算分子量 541 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC035499
基因名称 CCT5
Gene ID (NCBI) 22948
RRIDAB_2882643
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48643
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.