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  • Featured Product
  • KD/KO Validated

CCT5 Monoclonal antibody, PBS Only

CCT5 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1B12E10

Cat No : 67400-1-PBS

Print datasheet

Synonyms

CCT epsilon, CCT5, CCTE, KIAA0098, TCP 1 epsilon

验证数据展示

  • WB analysis using 67400-1-Ig (same clone as 67400-1-PBS)

    WB analysis using 67400-1-Ig (same clone as 67400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67400-1-Ig (CCT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67400-1-Ig (same clone as 67400-1-PBS)

    WB analysis of HEK-293 using 67400-1-Ig (same clone as 67400-1-PBS)

    WB result of CCT5 antibody (67400-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells. This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67400-1-Ig (same clone as 67400-1-PBS)

    IF Staining of U2OS using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

产品信息

67400-1-PBS targets CCT5 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen CCT5 fusion protein Ag21421 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Synonyms CCT epsilon, CCT5, CCTE, KIAA0098, TCP 1 epsilon
Calculated Molecular Weight 541 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC035499
Gene Symbol CCT5
Gene ID (NCBI) 22948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48643
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.