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CCT5 Recombinant antibody, PBS Only (Capture)

CCT5 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241510B3

Cat no : 84310-2-PBS

Print datasheet

Synonyms

KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon

验证数据展示

  • WB analysis using 84310-2-RR (same clone as 84310-2-PBS)

    WB analysis using 84310-2-RR (same clone as 84310-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84310-2-RR (CCT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84310-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01209-1

    Sandwich ELISA standard curve of MP01209-1

    Sandwich ELISA standard curve of MP01209-1, Human CCT5 Recombinant Matched Antibody Pair - PBS only. 84310-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2146. 84310-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

  • Sample test of MP01209-1

    Sample test of MP01209-1

    The mean CCT5 concentration was determined to be 28.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load and 35.60 ng/mL in HEK-293 cell extract based on a 2.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP01209-1

    Cytometric bead array standard curve of MP01209-1

    Cytometric bead array standard curve of MP01209-1, CCT5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84310-2-PBS. Detection antibody: 84310-1-PBS. Standard: Ag2146. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 84310-2-RR

    Affinity and Kinetic Characterization of 84310-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84310-2-RR against Human CCT5 were performed. The affinity constant is below 1 pM.

产品信息

84310-2-PBS targets CCT5 as part of a matched antibody pair:

MP01209-1: 84310-2-PBS capture and 84310-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen CCT5 fusion protein Ag2146 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Synonyms KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE, CCT epsilon
Calculated Molecular Weight 541 aa, 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC035499
Gene Symbol CCT5
Gene ID (NCBI) 22948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48643
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.