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CCT6B Monoclonal antibody, PBS Only

CCT6B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11A9

Cat no : 67961-1-PBS

Print datasheet

Synonyms

Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2

验证数据展示

  • WB analysis using 67961-1-Ig (same clone as 67961-1-PBS)

    WB analysis using 67961-1-Ig (same clone as 67961-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    FC experiment of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human CCT6B (67961-1-Ig, Clone:2F11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67961-1-Ig (same clone as 67961-1-PBS)

    IHC staining of mouse testis using 67961-1-Ig (same clone as 67961-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    IF Staining of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT6B antibody (67961-1-Ig, Clone: 2F11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

产品信息

67961-1-PBS targets CCT6B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CCT6B fusion protein Ag31295 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 6B (zeta 2)
Synonyms Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2
Calculated Molecular Weight 530 aa, 58 kDa
Observed Molecular Weight53-58 kDa
GenBank Accession NumberBC027591
Gene Symbol CCT6B
Gene ID (NCBI) 10693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92526
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.