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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67961-1-Ig (same clone as 67961-1-PBS)

    WB analysis using 67961-1-Ig (same clone as 67961-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67961-1-Ig (CCT6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    FC experiment of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human CCT6B (67961-1-Ig, Clone:2F11A9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67961-1-Ig (same clone as 67961-1-PBS)

    IHC staining of mouse testis using 67961-1-Ig (same clone as 67961-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67961-1-Ig (CCT6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    IF Staining of U2OS using 67961-1-Ig (same clone as 67961-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT6B antibody (67961-1-Ig, Clone: 2F11A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67961-1-PBS in a different storage buffer formulation.

CCT6B Monoclonal antibody, PBS Only

CCT6B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67961-1-PBS

产品说明书

CloneNo. 2F11A9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67961-1-PBS targets CCT6B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCT6B fusion protein Ag31295 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chaperonin containing TCP1, subunit 6B (zeta 2)
别名 Chaperonin containing T-complex polypeptide 1 subunit 6B, CCT-zeta-like, CCT-zeta-2, Cctz2, CCTZ 2
计算分子量 530 aa, 58 kDa
观测分子量53-58 kDa
GenBank蛋白编号BC027591
基因名称 CCT6B
Gene ID (NCBI) 10693
RRIDAB_2918712
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92526
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCT6B, also named as CCT-zeta-2, TCP-1-zeta-like, CCT-zeta-like, Testis-specific Tcp20 and Testis-specific protein TSA303, belongs to the TCP-1 chaperonin family. CCT6B is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin. CCT6B has 3 isoforms with the molecular mass of 53-58 kDa.