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  • Featured Product
  • KD/KO Validated

CCT8 Monoclonal antibody, PBS Only

CCT8 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1A7C3

Cat No : 67539-1-PBS

Print datasheet

Synonyms

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

验证数据展示

  • WB analysis using 67539-1-Ig (same clone as 67539-1-PBS)

    WB analysis using 67539-1-Ig (same clone as 67539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67539-1-Ig (CCT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

    IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

    IHC staining of human liver cancer using 67539-1-Ig (same clone as 67539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67539-1-Ig (CCT8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67539-1-Ig (same clone as 67539-1-PBS)

    IF Staining of MCF-7 using 67539-1-Ig (same clone as 67539-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCT8 antibody (67539-1-Ig, Clone: 1A7C3 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67539-1-Ig (same clone as 67539-1-PBS)

    WB analysis of HEK-293 using 67539-1-Ig (same clone as 67539-1-PBS)

    WB result of CCT8 antibody (67539-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells. This data was developed using the same antibody clone with 67539-1-PBS in a different storage buffer formulation.

产品信息

67539-1-PBS targets CCT8 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen CCT8 fusion protein Ag30082 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 8 (theta)
Synonyms C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta
Calculated Molecular Weight 548 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC012584
Gene Symbol CCT8
Gene ID (NCBI) 10694
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50990
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.