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  • Featured Product
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CD100 Monoclonal antibody

CD100 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 66582-1-Ig

Print datasheet

Synonyms

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

验证数据展示

  • WB analysis using 66582-1-Ig

    WB analysis using 66582-1-Ig

    various cells were subjected to SDS PAGE followed by western blot with 66582-1-Ig (CD100 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66582-1-Ig

    WB analysis of HeLa using 66582-1-Ig

    WB result of CD100 antibody (66582-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells.

  • IHC staining of human tonsillitis using 66582-1-Ig

    IHC staining of human tonsillitis using 66582-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66582-1-Ig

    IHC staining of human tonsillitis using 66582-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human lymphoma tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:225-1:900
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66582-1-Ig targets CD100 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD100 fusion protein Ag26461 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Synonyms A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC054500
Gene Symbol SEMA4D/CD100
Gene ID (NCBI) 10507
RRIDAB_2881942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

实验方案

Product Specific Protocols
WB protocol for CD100 antibody 66582-1-IgDownload protocol
IHC protocol for CD100 antibody 66582-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer

Authors - Xiang Liu
View Article