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Anti-Mouse Endoglin/CD105 (MJ7/18)

Endoglin/CD105 Monoclonal Antibody for IF-P, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-P, FC and More (1)

Conjugate

Unconjugated

CloneNo.

MJ7/18

Cat no : 65048-1-Ig

Print datasheet

Synonyms

CD105, S endoglin, MJ7/18, Eng, endoglin

验证数据展示

  • FC experiment of bEnd.3 using 65048-1-Ig

    FC experiment of bEnd.3 using 65048-1-Ig

    1X10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse CD105 (65048-1-Ig, Clone:MJ7/18) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.

  • IF Staining of mouse liver using 65048-1-Ig

    IF Staining of mouse liver using 65048-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD105 antibody (65048-1-Ig, Clone: MJ7/18 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IF-P detected inmouse liver tissue
Positive FC detected inbEnd.3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

65048-1-Ig targets Endoglin/CD105 in IF-P, FC applications and shows reactivity with mouse samples.

Tested Applications IF-P, FC Application Description
Cited ApplicationsIF
Tested Reactivity mouse
Cited Reactivitymouse
Immunogen Inflamed mouse skin 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name endoglin
Synonyms CD105, S endoglin, MJ7/18, Eng, endoglin
GenBank Accession NumberBC029080
Gene Symbol CD105
Gene ID (NCBI) 13805
RRIDAB_2918360
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ63961
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
IF protocol for Endoglin/CD105 antibody 65048-1-IgDownload protocol
FC protocol for Endoglin/CD105 antibody 65048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Bone

Radial extracorporeal shockwave promotes osteogenesis-angiogenesis coupling of bone marrow stromal cells from senile osteoporosis via activating the Piezo1/CaMKII/CREB axis

Authors - Bo Wang
View Article