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Anti-Human CD106 (1.G11B1)

CD106 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

1.G11B1

Cat no : 65049-1-Ig

Print datasheet

Synonyms

CD106, DKFZp779G2333, INCAM 100, V CAM 1, VCAM 1, VCAM1

验证数据展示

  • FC experiment of HUVEC using 65049-1-Ig

    FC experiment of HUVEC using 65049-1-Ig

    1X10^6 TNF-alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human CD106 (65049-1-Ig, Clone: 1.G11B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug mouse IgG1 isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65049-1-Ig targets CD106 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name vascular cell adhesion molecule 1
Synonyms CD106, DKFZp779G2333, INCAM 100, V CAM 1, VCAM 1, VCAM1
Calculated Molecular Weight 739 aa, 81 kDa
GenBank Accession NumberBC017276
Gene Symbol VCAM-1
Gene ID (NCBI) 7412
RRIDAB_2918361
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP19320
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
FC protocol for CD106 antibody 65049-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols