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CD117/c-Kit Recombinant antibody

CD117/c-Kit Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241313C5

Cat no : 84259-5-RR

Print datasheet

Synonyms

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

验证数据展示

  • WB analysis of Mo7e using 84259-5-RR

    WB analysis of Mo7e using 84259-5-RR

    Mo7e cells were subjected to SDS PAGE followed by western blot with 84259-5-RR (CD117/c-Kit antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84259-5-RR

    Affinity and Kinetic Characterization of 84259-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84259-5-RR against Human CD117/c-Kit were performed. The affinity constant is 0.90 nM.

经过测试的应用

Positive WB detected inMo7e cells
Planning an IHC experiment? We recommend our IHCeasy CD117/c-kit Ready-To-Use IHC Kit. CD117/c-kit primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84259-5-RR targets CD117/c-Kit in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Synonyms CD117, KIT, EC:2.7.10.1, c-Kit, C Kit
Calculated Molecular Weight 976 aa, 110 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC071593
Gene Symbol CD117
Gene ID (NCBI) 3815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10721
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

实验方案

Product Specific Protocols
WB protocol for CD117/c-Kit antibody 84259-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols