验证数据展示
经过测试的应用
| Positive WB detected in | MC/9 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83419-4-RR targets CD117/c-Kit in WB, ELISA applications and shows reactivity with Mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Mouse |
| Immunogen | Fusion Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | kit oncogene |
| Synonyms | Kit, CD117, c KIT, 240246B1 |
| Calculated Molecular Weight | 109 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | NM_001122733.1 |
| Gene Symbol | CD117 |
| Gene ID (NCBI) | 16590 |
| RRID | AB_3671068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P05532-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD117/c-Kit antibody 83419-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |