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CD11a/Integrin Alpha L Monoclonal antibody, PBS Only

CD11a/Integrin Alpha L Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3H12

Cat No : 66256-1-PBS

Print datasheet

Synonyms

CD11A, CD11a/Integrin Alpha L, CD11a/Integrin alpha-L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

验证数据展示

  • WB analysis of Ramos using 66256-1-Ig (same clone as 66256-1-PBS)

    WB analysis of Ramos using 66256-1-Ig (same clone as 66256-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

产品信息

66256-1-PBS targets CD11a/Integrin Alpha L in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CD11a/Integrin Alpha L fusion protein Ag7813 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Synonyms CD11A, CD11a/Integrin Alpha L, CD11a/Integrin alpha-L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol ITGAL
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20701
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).