• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31745-1-AP

    WB analysis using 31745-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31745-1-AP (CD11b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 31745-1-AP

    IF Staining of RAW 264.7 using 31745-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD11b antibody (31745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CD11b Polyclonal antibody

CD11b Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31745-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Ly 40, Itgam, integrin alpha M, CR3A, CR3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31745-1-AP targets CD11b in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen CD11b fusion protein Ag36145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin alpha M
Synonyms Ly 40, Itgam, integrin alpha M, CR3A, CR3
Calculated Molecular Weight127 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC156991
Gene Symbol CD11b
Gene ID (NCBI) 16409
RRIDAB_3670104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDE9Q604
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
WB protocol for CD11b antibody 31745-1-APDownload protocol
IF protocol for CD11b antibody 31745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols