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CD11b Polyclonal antibody

CD11b Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31745-1-AP

Print datasheet

Synonyms

Ly 40, Itgam, integrin alpha M, CR3A, CR3

验证数据展示

  • WB analysis using 31745-1-AP

    WB analysis using 31745-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31745-1-AP (CD11b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJ774A.1 cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31745-1-AP targets CD11b in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen CD11b fusion protein Ag36145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin alpha M
Synonyms Ly 40, Itgam, integrin alpha M, CR3A, CR3
Calculated Molecular Weight127 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC156991
Gene Symbol CD11b
Gene ID (NCBI) 16409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDE9Q604
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
WB protocol for CD11b antibody 31745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols