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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65568-1-PBS

    FC experiment of human PBMCs using 65568-1-PBS

    1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD11c (3.9) Mouse IgG2a Recombinant Antibody, PBS Only (65568-1-PBS, Clone:3.9) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were not fixed.

  • FC experiment of human peripheral blood leukocyte using 65568-1-MR (same clone as 65568-1-PBS)

    FC experiment of human peripheral blood leukocyte using 65568-1-MR (same clone as 65568-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65568-1-MR, Clone: 3.9) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65568-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocyte using 65568-1-MR (same clone as 65568-1-PBS)

    FC experiment of human peripheral blood leukocyte using 65568-1-MR (same clone as 65568-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65568-1-MR, Clone: 3.9) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65568-1-PBS in a different storage buffer formulation.

Anti-Human CD11c (3.9) Mouse IgG2a Recombinant Antibody, PBS Only

CD11c Uni-rAbTM Recombinant Antibody for FC
Cat No. 65568-1-PBS

产品说明书

CloneNo. 3.9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65568-1-PBS targets CD11c in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha X (complement component 3 receptor 4 subunit)
别名 Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C
计算分子量 1169 aa, 129 kDa
GenBank蛋白编号BC038237
基因名称 CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CD11c antibody 65568-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols