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CD122/IL-2RB Monoclonal antibody, PBS Only (Detector)

CD122/IL-2RB Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12C7

Cat no : 60174-1-PBS

Print datasheet

Synonyms

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

验证数据展示

  • WB analysis using 60174-1-Ig (same clone as 60174-1-PBS)

    WB analysis using 60174-1-Ig (same clone as 60174-1-PBS)

    Untreated and PNGase F-treated lysates of NK-92 cells were subjected to SDS PAGE followed by western blot with 60174-1-Ig (CD122/IL2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60174-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50451-1

    Cytometric bead array standard curve of MP50451-1

    Cytometric bead array standard curve of MP50451-1, CD122/IL2RB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60174-3-PBS. Detection antibody: 60174-1-PBS. Standard:Ag3929. Range: 0.098-25 ng/mL.

  • Cytometric bead array standard curve of MP50451-2

    Cytometric bead array standard curve of MP50451-2

    Cytometric bead array standard curve of MP50451-2, CD122/IL2RB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60174-2-PBS. Detection antibody: 60174-1-PBS. Standard:Ag3929. Range: 0.098-50 ng/mL.

产品信息

60174-1-PBS targets CD122/IL-2RB as part of a matched antibody pair:

MP50451-1: 60174-3-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)

MP50451-2: 60174-2-PBS capture and 60174-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD122/IL-2RB fusion protein Ag3929 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 2 receptor, beta
Synonyms IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta
Calculated Molecular Weight 551 aa, 61 kDa
Observed Molecular Weight75 kDa, 61 kDa
GenBank Accession NumberBC025691
Gene Symbol IL2RB
Gene ID (NCBI) 3560
ENSEMBL Gene IDENSG00000100385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14784
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.