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验证数据展示

  • FC experiment of human peripheral blood mononuclear cells using PE-65159

    FC experiment of human peripheral blood mononuclear cells using PE-65159

    1X10^6 PHA-stimulated (5 μg/mL, 3 days) (red) or unstimulated (blue) human peripheral blood mononuclear cells (PBMCs) were surface stained with 10.00 ul PE Anti-Human CD137 (PE-65159, Clone: 4B4-1). Cells were not fixed.

PE Anti-Human CD137 (4B4-1)

CD137 Monoclonal Antibody for FC
Cat No. PE-65159

产品说明书

CloneNo. 4B4-1

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human, Non-human primates

应用

FC

别名

4 1BB, 4 1BB ligand receptor, CD137, CDw137, ILA, T cell antigen 4 1BB homolog, T cell antigen ILA, TNFRSF9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood mononuclear cells

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65159 targets CD137 in FC applications and shows reactivity with Human, Non-human primates samples.

经测试应用 FC Application Description
经测试反应性 Human, Non-human primates
免疫原 Recombinant human CD137 ectodomain 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 9
别名 4 1BB, 4 1BB ligand receptor, CD137, CDw137, ILA, T cell antigen 4 1BB homolog, T cell antigen ILA, TNFRSF9
计算分子量 28 kDa
GenBank蛋白编号BC006196
基因名称 TNFRSF9/CD137
Gene ID (NCBI) 3604
RRIDAB_2883937
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ07011
储存缓冲液 PBS with 0.1% sodium azide.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for PE CD137 antibody PE-65159Download protocol
Standard Protocols
Click here to view our Standard Protocols