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CD14 Monoclonal antibody, PBS Only

CD14 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1D9

Cat No : 60253-1-PBS

Print datasheet

Synonyms

CD14, CD14 molecule

验证数据展示

  • WB analysis of THP-1 using 60253-1-Ig (same clone as 60253-1-PBS)

    WB analysis of THP-1 using 60253-1-Ig (same clone as 60253-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD14 antibody included
  • IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD14 antibody included
  • IF Staining of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

    IF Staining of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

产品信息

60253-1-PBS targets CD14 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD14 fusion protein Ag10693 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD14 molecule
Synonyms CD14, CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.