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验证数据展示

  • WB analysis of HL-60 using 83055-6-RR (same clone as 83055-6-PBS)

    WB analysis of HL-60 using 83055-6-RR (same clone as 83055-6-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83055-6-RR (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83055-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83055-6-RR

    Affinity and Kinetic Characterization of 83055-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83055-6-RR against Human CD14 were performed. The affinity constant is 1.43 nM.

CD14 Recombinant antibody, PBS Only

CD14 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83055-6-PBS

产品说明书

CloneNo. 230332D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

产品信息

83055-6-PBS targets CD14 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD14 molecule
别名 Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 230332D4
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08571
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.