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Mouse CD14 Recombinant antibody, PBS Only (Detector)

CD14 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240225A8

Cat no : 83297-1-PBS

Print datasheet

Synonyms

CD14 antigen, 240225A8

验证数据展示

  • Sandwich ELISA standard curve of MP00313-4

    Sandwich ELISA standard curve of MP00313-4

    Sandwich ELISA standard curve of MP00313-4, Mouse CD14 Monoclonal Matched Antibody Pair - PBS only. 83297-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0847. 83297-1-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00313-4

    Sample test of MP00313-4

    Mouse splenocytes (1 x 10^6 cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS.The mean CD-14 concentration was determined to be 2,187.4 pg/mL in unstimulated spleen, 8,939.2 pg/mL in IFN-γ and LPS stimulated spleen supernatant.

  • Sample test of MP00313-4

    Sample test of MP00313-4

    Serum of sixteen mice was measured. The CD14 concentration of detected samples was determined to be 113.29 ng/mL with a range of 83.24-160.50 ng/mL.

  • Cytometric bead array standard curve of MP00313-1

    Cytometric bead array standard curve of MP00313-1

    Cytometric bead array standard curve of MP00313-1, MOUSE CD14 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83297-3-PBS. Detection antibody: 83297-1-PBS. Standard: Eg0847. Range: 1.25-40 ng/mL.

产品信息

83297-1-PBS targets CD14 as part of a matched antibody pair:

MP00313-1: 83297-3-PBS capture and 83297-1-PBS detection (validated in Cytometric bead array)

MP00313-4: 83297-7-PBS capture and 83297-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD14 antigen
Synonyms CD14 antigen, 240225A8
Calculated Molecular Weight39 kDa
GenBank Accession NumberNM_009841.4
Gene Symbol Cd14
Gene ID (NCBI) 12475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10810
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C