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CD146/MCAM Monoclonal antibody, PBS Only

CD146/MCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D8A9

Cat no : 66153-1-PBS

Print datasheet

Synonyms

CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

验证数据展示

  • WB analysis of A375 using 66153-1-Ig (same clone as 66153-1-PBS)

    WB analysis of A375 using 66153-1-Ig (same clone as 66153-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66153-1-Ig (same clone as 66153-1-PBS)

    WB analysis of HepG2 using 66153-1-Ig (same clone as 66153-1-PBS)

    Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD146/MCAM antibody included
  • IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD146/MCAM antibody included
  • IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66153-1-Ig (same clone as 66153-1-PBS)

    IF Staining of HUVEC using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.

产品信息

66153-1-PBS targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD146/MCAM fusion protein Ag11855 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name melanoma cell adhesion molecule
Synonyms CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC056418
Gene Symbol CD146
Gene ID (NCBI) 4162
RRIDAB_2881549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43121
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.