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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82909-7-RR (same clone as 82909-7-PBS)

    WB analysis using 82909-7-RR (same clone as 82909-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82909-7-RR (CD147 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

    IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

    IF Staining of HeLa using 82909-7-RR (same clone as 82909-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82909-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82909-7-RR

    Affinity and Kinetic Characterization of 82909-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82909-7-RR against Human CD147 were performed. The affinity constant is 21.7 nM.

CD147 Recombinant antibody, PBS Only

CD147 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82909-7-PBS

产品说明书

CloneNo. 230046A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD147/BSG Ready-To-Use IHC Kit. CD147/BSG primary antibody included.

产品信息

82909-7-PBS targets CD147 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 basigin (Ok blood group)
别名 EMMPRIN, Collagenase stimulatory factor, BSG, Basigin, 5F7
计算分子量 385 aa, 42 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC009040
基因名称 CD147
Gene ID (NCBI) 682
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP35613
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).