• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CD151 Monoclonal antibody, PBS Only

CD151 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB,IP,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

2A8G8

Cat No : 66567-1-PBS

Print datasheet

Synonyms

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24

验证数据展示

  • WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

    WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD151 antibody included
  • IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD151 antibody included
  • IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

    IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

    IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:10000) with human placenta tissue lysate 800 ug. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 66567-1-Ig (same clone as 66567-1-PBS)

    FC experiment of A549 using 66567-1-Ig (same clone as 66567-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CD151 (66567-1-Ig, Clone:2A8G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD151 Ready-To-Use IHC Kit. CD151 primary antibody included.

产品信息

66567-1-PBS targets CD151 in WB, IP, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,IP,Indirect ELISA,IHC,FC Application Description
Tested Reactivity Human
Immunogen CD151 fusion protein Ag25985 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD151 molecule (Raph blood group)
Synonyms CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC001374
Gene Symbol CD151
Gene ID (NCBI) 977
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48509
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.