FITC Anti-Human CD154 (24-31)

CD154 Monoclonal Antibody for FC
Cat No. FITC-65156

产品说明书

CloneNo. 24-31

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human, Non-human primates

应用

FC

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -


经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65156 targets CD154 in FC applications and shows reactivity with Human, Non-human primates samples.

经测试应用 FC Application Description
经测试反应性 Human, Non-human primates
免疫原 human CD154 fusion protein 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD40 ligand
别名 CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP
计算分子量 261 aa, 29 kDa
GenBank蛋白编号BC071754
基因名称 CD40 Ligand
Gene ID (NCBI) 959
RRIDAB_2920500
偶联类型 FITC Fluorescent Dye
最大激发/发射波长498 nm / 526 nm
形式 Liquid
纯化方式The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP29965
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

实验方案

Product Specific Protocols
FC protocol for FITC CD154 antibody FITC-65156Download protocol
Standard Protocols
Click here to view our Standard Protocols
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