验证数据展示
经过测试的应用
Positive IHC detected in | human liver tissue, human placenta tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16559-1-AP targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, IF-P, ELISA Application Description |
文献引用应用 | IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat |
免疫原 | CD16 fusion protein Ag9787 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
别名 | Fc gamma RIIIa, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen |
计算分子量 | 254 aa, 29 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC017865 |
基因名称 | CD16a |
Gene ID (NCBI) | 2214 |
ENSEMBL Gene ID | ENSG00000203747 |
RRID | AB_2878279 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P08637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
实验方案
Product Specific Protocols | |
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IHC protocol for CD16 antibody 16559-1-AP | Download protocol |
IF protocol for CD16 antibody 16559-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Immunother Cancer Discovery of a glucocorticoid receptor (GR) activity signature correlates with immune cell infiltration in adrenocortical carcinoma | ||
J Allergy Clin Immunol Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2. | ||
Theranostics Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring. | ||
Theranostics Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model. | ||
J Transl Med Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease | ||
Front Immunol Efficacy of MUC1-targeted CAR-NK cells against human tongue squamous cell carcinoma |