CD16 Polyclonal antibody

CD16 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 16559-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

IHC, IF-P, ELISA and More (1)

Fc gamma RIIIa, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman liver tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16559-1-AP targets CD16 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 CD16 fusion protein Ag9787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 Fc gamma RIIIa, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen
计算分子量 254 aa, 29 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_2878279
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.

实验方案

Product Specific Protocols
IHC protocol for CD16 antibody 16559-1-APDownload protocol
IF protocol for CD16 antibody 16559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Immunother Cancer

Discovery of a glucocorticoid receptor (GR) activity signature correlates with immune cell infiltration in adrenocortical carcinoma

Authors - Kan Wu
humanWB

J Allergy Clin Immunol

Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.

Authors - Yunsheng Liang
ratIF

Theranostics

Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring.

Authors - Luodan Yang
ratWB,IF

Theranostics

Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.

Authors - Luodan Yang
humanIF

J Transl Med

Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease

Authors - Beibei Li
mouseIHC

Front Immunol

Efficacy of MUC1-targeted CAR-NK cells against human tongue squamous cell carcinoma

Authors - Xiaolan Lin
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