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CD16 Recombinant antibody, PBS Only

CD16 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230337G12

Cat no : 83110-1-PBS

Print datasheet

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

验证数据展示

  • WB analysis of human placenta using 83110-1-RR (same clone as 83110-1-PBS)

    WB analysis of human placenta using 83110-1-RR (same clone as 83110-1-PBS)

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83110-1-RR (CD16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83110-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83110-1-RR

    Affinity and Kinetic Characterization of 83110-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83110-1-RR against Human CD16 were performed. The affinity constant is 71 nM.

Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

83110-1-PBS targets CD16 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Synonyms Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC017865
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.