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验证数据展示

  • WB analysis of human placenta using 83110-1-RR (same clone as 83110-1-PBS)

    WB analysis of human placenta using 83110-1-RR (same clone as 83110-1-PBS)

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83110-1-RR (CD16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83110-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83110-1-RR

    Affinity and Kinetic Characterization of 83110-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83110-1-RR against Human CD16 were performed. The affinity constant is 71 nM.

CD16 Recombinant antibody, PBS Only

CD16 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83110-1-PBS

产品说明书

CloneNo. 230337G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

产品信息

83110-1-PBS targets CD16 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A
计算分子量 254 aa, 29 kDa
观测分子量55 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.