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CD163 Polyclonal antibody

CD163 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 31024-1-AP

Print datasheet

Synonyms

sCD163, M130

验证数据展示

  • WB analysis using 31024-1-AP

    WB analysis using 31024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31024-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 31024-1-AP

    IHC staining of mouse spleen using 31024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31024-1-AP

    IHC staining of mouse spleen using 31024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 31024-1-AP

    IF Staining of mouse testis using 31024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CD163 antibody (31024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 31024-1-AP

    IF Staining of mouse liver using 31024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CD163 antibody (31024-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue, mouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31024-1-AP targets CD163 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD163 antigen
Synonyms sCD163, M130
Calculated Molecular Weight121 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberNM_053094.2
Gene Symbol Cd163
Gene ID (NCBI) 93671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2VLH6-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
WB protocol for CD163 antibody 31024-1-APDownload protocol
IHC protocol for CD163 antibody 31024-1-APDownload protocol
IF protocol for CD163 antibody 31024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols