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Anti-Human CD177 Rabbit Recombinant Antibody, PBS Only

CD177 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241339F4

Cat no : 98086-1-PBS

Print datasheet

Synonyms

NB1, Human neutrophil alloantigen 2a, HNA-2a, HNA2A, HNA 2a

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

    FC experiment of human peripheral blood leukocytes using 98086-1-RR (same clone as 98086-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD177 Rabbit Recombinant Antibody (98086-1-RR, Clone:241339F4) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98086-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98086-1-RR

    Affinity and Kinetic Characterization of 98086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98086-1-RR against Human CD177 were performed. The affinity constant is 1.70 nM.

产品信息

98086-1-PBS targets CD177 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD177 molecule
Synonyms NB1, Human neutrophil alloantigen 2a, HNA-2a, HNA2A, HNA 2a
Calculated Molecular Weight 437 aa, 46 kDa
GenBank Accession NumberBC029167
Gene Symbol CD177
Gene ID (NCBI) 57126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8N6Q3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).