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CoraLite®594 Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

TS1/18

Cat no : CL594-65190

Print datasheet

Synonyms

ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2

验证数据展示

  • FC experiment of human PBMCs using CL594-65190

    FC experiment of human PBMCs using CL594-65190

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD18 (CL594-65190, Clone:TS1/18) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65190-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65190 targets CD18 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human Beta 2 Integrin 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Synonyms ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2
Calculated Molecular Weight 85 kDa
GenBank Accession NumberBC005861
Gene Symbol CD18
Gene ID (NCBI) 3689
ENSEMBL Gene IDENSG00000160255
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05107
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

实验方案

Product Specific Protocols
FC protocol for CL594 CD18 antibody CL594-65190Download protocol
Standard Protocols
Click here to view our Standard Protocols