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CD1d Polyclonal antibody

CD1d Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17336-1-AP

Print datasheet

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

验证数据展示

  • WB analysis of Jurkat cells using 17336-1-AP

    WB analysis of Jurkat cells using 17336-1-AP

    WB result of CD1D antibody (17336-1-AP, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

  • WB analysis of mouse skin using 17336-1-AP

    WB analysis of mouse skin using 17336-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17336-1-AP (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skin tissue, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17336-1-AP is CD1d Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitypig
Immunogen CD1d fusion protein Ag11371 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD1d molecule
Synonyms CD1A, CD1D, CD1d molecule, R3, R3G1
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 38-40 kDa, 50-55 kDa
GenBank Accession NumberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
RRIDAB_2878387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-50 kDa.

实验方案

Product Specific Protocols
WB protocol for CD1d antibody 17336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB,IF

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article