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CD2 Polyclonal antibody

CD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23973-1-AP

Print datasheet

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

验证数据展示

  • WB analysis of Jurkat using 23973-1-AP

    WB analysis of Jurkat using 23973-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23973-1-AP (CD2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 23973-1-AP

    IHC staining of human tonsillitis using 23973-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23973-1-AP (CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • IHC staining of human tonsillitis using 23973-1-AP

    IHC staining of human tonsillitis using 23973-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23973-1-AP (CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • IF Staining of Jurkat using 23973-1-AP

    IF Staining of Jurkat using 23973-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 23973-1-AP (CD2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23973-1-AP is CD2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD2 fusion protein Ag21122 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD2 molecule
Synonyms CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_2879384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP06729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
WB protocol for CD2 antibody 23973-1-APDownload protocol
IHC protocol for CD2 antibody 23973-1-APDownload protocol
IF protocol for CD2 antibody 23973-1-APDownload protocol
FC protocol for CD2 antibody 23973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols