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CD2 Polyclonal antibody

CD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31803-1-AP

Print datasheet

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

验证数据展示

  • WB analysis using 31803-1-AP

    WB analysis using 31803-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 31803-1-AP (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 31803-1-AP

    IHC staining of human tonsillitis using 31803-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31803-1-AP (CD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 31803-1-AP

    IHC staining of human tonsillitis using 31803-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31803-1-AP (CD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 31803-1-AP

    IF Staining of Jurkat using 31803-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD2 antibody (31803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31803-1-AP targets CD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD2 molecule
Synonyms LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP06729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
WB protocol for CD2 antibody 31803-1-APDownload protocol
IHC protocol for CD2 antibody 31803-1-APDownload protocol
IF protocol for CD2 antibody 31803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols