验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31803-1-AP targets CD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | CD2 molecule |
别名 | LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor |
计算分子量 | 351 aa, 39 kDa |
观测分子量 | 45-50 kDa |
GenBank蛋白编号 | BC033583 |
基因名称 | CD2 |
Gene ID (NCBI) | 914 |
ENSEMBL Gene ID | ENSG00000116824 |
RRID | AB_3670119 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | P06729 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
实验方案
Product Specific Protocols | |
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WB protocol for CD2 antibody 31803-1-AP | Download protocol |
IHC protocol for CD2 antibody 31803-1-AP | Download protocol |
IF protocol for CD2 antibody 31803-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |