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验证数据展示

  • WB analysis using 84108-5-RR

    WB analysis using 84108-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 84108-5-RR

    IF Staining of Jurkat using 84108-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells (positive cell) and HeLa cell (negative cell) using CD2 antibody (84108-5-RR, Clone: 241222B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84108-5-RR

    Affinity and Kinetic Characterization of 84108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

CD2 Recombinant antibody

CD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84108-5-RR

产品说明书

CloneNo. 241222B8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, NK-92 cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84108-5-RR targets CD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD2 fusion protein Eg1271 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD2 molecule
别名 241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor
计算分子量 351 aa, 39 kDa
观测分子量50 kDa
GenBank蛋白编号BC033583
基因名称 CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_3671675
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
WB protocol for CD2 antibody 84108-5-RRDownload protocol
IF protocol for CD2 antibody 84108-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols