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CD200 Polyclonal antibody

CD200 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31444-1-AP

Print datasheet

Synonyms

OX2, Mox2, CD200 antigen

验证数据展示

  • WB analysis using 31444-1-AP

    WB analysis using 31444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31444-1-AP (CD200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 31444-1-AP

    IHC staining of mouse spleen using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31444-1-AP

    IHC staining of mouse spleen using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse lung tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue
Positive IHC detected inmouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31444-1-AP targets CD200 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD200 antigen
Synonyms OX2, Mox2, CD200 antigen
Calculated Molecular Weight31 kDa
Observed Molecular Weight40-50 kDa
Gene Symbol Cd200
Gene ID (NCBI) 17470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO54901
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

实验方案

Product Specific Protocols
WB protocol for CD200 antibody 31444-1-APDownload protocol
IHC protocol for CD200 antibody 31444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols