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Anti-Mouse CD200 (OX90)

CD200 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX90

Cat no : 65230-1-Ig

Print datasheet

Synonyms

Cd200, CD200 antigen, Mox2, OX2

验证数据展示

  • FC experiment of mouse splenocytes using 65230-1-Ig

    FC experiment of mouse splenocytes using 65230-1-Ig

    1x10^6 mouse splenocytes were surface stained with 1 ug Anti-Mouse CD200 (65230-1-Ig, Clone: OX90) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 1 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.

  • FC experiment of mouse splenocytes using 65230-1-Ig

    FC experiment of mouse splenocytes using 65230-1-Ig

    1x10^6 mouse splenocytes were surface stained with 1 ug Anti-Mouse CD200 (65230-1-Ig, Clone: OX90) and FITC anti-rat IgG2a Antibody at dilution 1:100, or 1 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65230-1-Ig targets CD200 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse CD200-rat CD4 fusion protein 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD200 antigen
Synonyms Cd200, CD200 antigen, Mox2, OX2
GenBank Accession NumberBC051984
Gene Symbol Cd200
Gene ID (NCBI) 17470
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ80VX2
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

实验方案

Product Specific Protocols
FC protocol for CD200 antibody 65230-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols