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CD21 Polyclonal antibody

CD21 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28206-1-AP

Print datasheet

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

验证数据展示

  • IHC staining of human tonsillitis using 28206-1-AP

    IHC staining of human tonsillitis using 28206-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28206-1-AP (CD21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28206-1-AP

    IHC staining of human tonsillitis using 28206-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28206-1-AP (CD21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 28206-1-AP

    IF Staining of human tonsillitis using 28206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD21 antibody (28206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 28206-1-AP

    IF Staining of human tonsillitis using 28206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD21 antibody (28206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28206-1-AP targets CD21 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity Human
Immunogen CD21 fusion protein Ag27971 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component (3d/Epstein Barr virus) receptor 2
Synonyms C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
Calculated Molecular Weight 1092 aa, 119 kDa
GenBank Accession NumberBC136394
Gene Symbol CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2881087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
IHC protocol for CD21 antibody 28206-1-APDownload protocol
IF protocol for CD21 antibody 28206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols