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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65512-1-PBS

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65512-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65512-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65512-1-PBS

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Antibody, PBS Only

CD21 Recombinant Antibody for FC
Cat No. 65512-1-PBS

产品说明书

CloneNo. BU32

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

EBV receptor, CR2, Complement receptor type 2, Complement C3d receptor, C3DR

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65512-1-PBS targets CD21 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Recombinant
产品类型 Antibody
全称 complement component (3d/Epstein Barr virus) receptor 2
别名 EBV receptor, CR2, Complement receptor type 2, Complement C3d receptor, C3DR
计算分子量 1092 aa, 119 kDa
GenBank蛋白编号BC136394
基因名称 CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP20023
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
FC protocol for CD21 antibody 65512-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols