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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 65512-1-PBS

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65512-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    FC experiment of human PBMCs using 65512-1-MR (same clone as 65512-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65512-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65512-1-PBS

    FC experiment of human PBMCs using 65512-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-PBS, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Antibody, PBS Only

CD21 Recombinant Antibody for FC
Cat No. 65512-1-PBS

Print datasheet

CloneNo. BU32

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Synonyms

EBV receptor, CR2, Complement receptor type 2, Complement C3d receptor, C3DR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65512-1-PBS targets CD21 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name complement component (3d/Epstein Barr virus) receptor 2
Synonyms EBV receptor, CR2, Complement receptor type 2, Complement C3d receptor, C3DR
Calculated Molecular Weight 1092 aa, 119 kDa
GenBank Accession NumberBC136394
Gene Symbol CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP20023
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
FC protocol for CD21 antibody 65512-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols