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验证数据展示

  • WB analysis using 84565-2-RR (same clone as 84565-2-PBS)

    WB analysis using 84565-2-RR (same clone as 84565-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

    IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

    IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01407-1

    Cytometric bead array standard curve of MP01407-1

    Cytometric bead array standard curve of MP01407-1, MOUSE CD21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84565-2-PBS. Detection antibody: 84565-3-PBS. Standard: Eg1396. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84565-2-RR

    Affinity and Kinetic Characterization of 84565-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84565-2-RR against Mouse CD21/CD35 were performed. The affinity constant is 4.84 nM.

Mouse CD21/CD35 Recombinant antibody, PBS Only (Capture)

CD21/CD35 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 84565-2-PBS

产品说明书

CloneNo. 241957E6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

CD21, Cr2, 241957E6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84565-2-PBS targets CD21/CD35 as part of a matched antibody pair:

MP01407-1: 84565-2-PBS capture and 84565-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement receptor 2
别名 CD21, Cr2, 241957E6
计算分子量113kDa
观测分子量160-210 kDa
GenBank蛋白编号AAA37448.1
基因名称 Cr2
Gene ID (NCBI) 12902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19070
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 short consensus repeats (SCRs) (PMID: 6230668; 2551147). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, binds C3b and C4b. The murine Cr2 gene encodes for both CD21 and CD35 (PMID: 2139460; 19559484). Mouse CD35 comprises 21 SCRs while mouse CD21 is identical to mouse CD35 except that it lacks the first 6 SCRs. CD21/CD35 forms trimolecular complexes with CD19 and CD81 and plays roles in the regulation of humoral immune responses (PMID: 11043778).