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CD22 Polyclonal antibody

CD22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30954-1-AP

Print datasheet

Synonyms

Sialic acid-binding Ig-like lectin 2, CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22

验证数据展示

  • WB analysis using 30954-1-AP

    WB analysis using 30954-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30954-1-AP (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 30954-1-AP

    IHC staining of human tonsillitis using 30954-1-AP

    Immunohistochemical analysis of paraffin-embedded tonsillitis slide using 30954-1-AP (CD22 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD22 antibody included
  • IHC staining of human tonsillitis using 30954-1-AP

    IHC staining of human tonsillitis using 30954-1-AP

    Immunohistochemical analysis of paraffin-embedded tonsillitis slide using 30954-1-AP (CD22 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD22 antibody included

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30954-1-AP targets CD22 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD22 molecule
Synonyms Sialic acid-binding Ig-like lectin 2, CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22
Calculated Molecular Weight95kd
Observed Molecular Weight120-130 kDa
GenBank Accession NumberNM_001771.4
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

实验方案

Product Specific Protocols
WB protocol for CD22 antibody 30954-1-APDownload protocol
IHC protocol for CD22 antibody 30954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols