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CD22 Recombinant antibody, PBS Only

CD22 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230550C11

Cat no : 83303-1-PBS

Print datasheet

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

验证数据展示

  • WB analysis using 83303-1-RR (same clone as 83303-1-PBS)

    WB analysis using 83303-1-RR (same clone as 83303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83303-1-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

    IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

    IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD22 antibody included
  • Affinity and Kinetic Characterization of 83303-1-RR

    Affinity and Kinetic Characterization of 83303-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83303-1-RR against Human CD22 were performed. The affinity constant is 3.33 nM.

Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

产品信息

83303-1-PBS targets CD22 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD22 molecule
Synonyms CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22
Calculated Molecular Weight95kd
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001771.4
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20273
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.