CD22 Recombinant antibody
CD22 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241314G10
验证数据展示
经过测试的应用
Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84265-2-RR targets CD22 in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Recombinant Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | CD22 molecule |
Synonyms | CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22 |
Calculated Molecular Weight | 847 aa, 95 kDa |
Observed Molecular Weight | 130-140 kDa |
GenBank Accession Number | BC109306 |
Gene Symbol | CD22 |
Gene ID (NCBI) | 933 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P20273 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD22 antibody 84265-2-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |