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Anti-Human CD226 (11A8)

CD226 Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

11A8

Cat no : 65247-1-Ig

Print datasheet

Synonyms

CD226, CD226 antigen, CD226 molecule, DNAM 1, DNAM1, DNAX accessory molecule 1, PTA1, TLiSA1

验证数据展示

  • FC experiment of human PBMCs using 65247-1-Ig

    FC experiment of human PBMCs using 65247-1-Ig

    1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 0.2 ug Anti-Human CD226 (65247-1-Ig, Clone:11A8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65247-1-Ig

    FC experiment of human PBMCs using 65247-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 (65247-1-Ig, Clone:11A8) and APC-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65247-1-Ig targets CD226 in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA Application Description
Tested Reactivity Human
Immunogen Human NK Cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD226 molecule
Synonyms CD226, CD226 antigen, CD226 molecule, DNAM 1, DNAM1, DNAX accessory molecule 1, PTA1, TLiSA1
Calculated Molecular Weight 336 aa, 39 kDa
GenBank Accession NumberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ15762
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

实验方案

Product Specific Protocols
FC protocol for CD226 antibody 65247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols