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验证数据展示

  • WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

    WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

    IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82991-3-RR (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

    IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using CD226 antibody (82991-3-RR, Clone: 230041E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

CD226 Recombinant antibody, PBS Only

CD226 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82991-3-PBS

产品说明书

CloneNo. 230041E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82991-3-PBS targets CD226 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD226 molecule
别名 DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen
计算分子量 336 aa, 39 kDa
观测分子量65 kDa
GenBank蛋白编号BC074787
基因名称 CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ15762
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).