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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

    WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

    IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82991-3-RR (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

    IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using CD226 antibody (82991-3-RR, Clone: 230041E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

CD226 Recombinant antibody, PBS Only

CD226 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82991-3-PBS
CloneNo. 230041E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82991-3-PBS targets CD226 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD226 molecule
Synonyms DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen
Calculated Molecular Weight 336 aa, 39 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15762
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).