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CD23 Polyclonal antibody

CD23 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10300-1-AP

Print datasheet

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

验证数据展示

  • IHC staining of human tonsillitis using 10300-1-AP

    IHC staining of human tonsillitis using 10300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10300-1-AP (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 10300-1-AP

    IHC staining of human tonsillitis using 10300-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10300-1-AP (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 10300-1-AP is CD23 Fusion Protein expressed in E. coli.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen CD23 fusion protein Ag0425 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor
Calculated Molecular Weight 321 aa, 36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.