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CD23 Polyclonal antibody

CD23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18642-1-AP

Print datasheet

Synonyms

Fc epsilon RII, C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2

验证数据展示

  • IHC staining of human tonsillitis using 18642-1-AP

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (CD23 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 18642-1-AP

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (CD23 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • WB analysis of Raji using 18642-1-AP

    WB analysis of Raji using 18642-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18642-1-AP targets CD23 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CD23 fusion protein Ag13348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms Fc epsilon RII, C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC014108
Gene Symbol CD23
Gene ID (NCBI) 2208
RRIDAB_2878555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

实验方案

Product Specific Protocols
WB protocol for CD23 antibody 18642-1-APDownload protocol
IHC protocol for CD23 antibody 18642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols