• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD23 Recombinant antibody

CD23 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241269B10

Cat no : 84024-2-RR

Print datasheet

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

验证数据展示

  • WB analysis using 84024-2-RR

    WB analysis using 84024-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84024-2-RR (CD23,FCER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84024-2-RR

    IHC staining of human tonsillitis using 84024-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 84024-2-RR

    IHC staining of human tonsillitis using 84024-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD23 antibody included
  • Affinity and Kinetic Characterization of 84024-2-RR

    Affinity and Kinetic Characterization of 84024-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83996-4-RR against Human CD23 were performed. The affinity constant is 3.66 nM.

经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells, U-937 cells, NIH/3T3 cells, RAW 264.7 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84024-2-RR targets CD23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2
Calculated Molecular Weight 321 aa, 36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells

实验方案

Product Specific Protocols
WB protocol for CD23 antibody 84024-2-RRDownload protocol
IHC protocol for CD23 antibody 84024-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols