验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Ramos cells, Daudi cells, U-937 cells, NIH/3T3 cells, RAW 264.7 cells, mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84024-2-RR targets CD23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Fc fragment of IgE, low affinity II, receptor for (CD23) |
| 别名 | C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2 |
| 计算分子量 | 321 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC064417 |
| 基因名称 | CD23 |
| Gene ID (NCBI) | 2208 |
| RRID | AB_3671593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06734 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD23 antibody 84024-2-RR | Download protocol |
| IHC protocol for CD23 antibody 84024-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |