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验证数据展示

  • WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01134-2

    Sandwich ELISA standard curve of MP01134-2

    Sandwich ELISA standard curve of MP01134-2, Mouse CD23 Recombinant Matched Antibody Pair - PBS only. 84212-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1295. 84212-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01134-2

    Sample test of MP01134-2

    Serum of six mouse was measured. The CD23 concentration of detected samples was determined to be 2624.00 pg/mL with a range of 1279.00 - 7445.00 pg/mL.

  • Affinity and Kinetic Characterization of 84212-3-RR

    Affinity and Kinetic Characterization of 84212-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

Mouse CD23/Fc epsilon RII Recombinant antibody, PBS Only (Detector)

CD23/Fc epsilon RII Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84212-3-PBS

产品说明书

CloneNo. 241385C9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, Sandwich ELISA, Indirect ELISA, Sample test

别名

241385C9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84212-3-PBS targets CD23/Fc epsilon RII as part of a matched antibody pair:

MP01134-2: 84212-4-PBS capture and 84212-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc receptor, IgE, low affinity II, alpha polypeptide
别名 241385C9
计算分子量38kDa
观测分子量46 kDa
GenBank蛋白编号EDL21948.1
基因名称 Fcer2a
Gene ID (NCBI) 14128
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP20693-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.